cell culture c2c12 myoblast cell line  (ATCC)

Journal: Applied Materials Today

Article Title: A biosynthesized gold nanoparticle from Staphylococcus aureus – as a functional factor in muscle tissue engineering

doi: 10.1016/j.apmt.2020.100905

Figure Lengend Snippet: Fig. 4. The purified AuNPs exhibted excellent biocompatibility for muscle cells. (a-b) After C2C12 cells (a) or CMs (b) were treated with pristine AuNPs, purified AuNPs and supernatant for 12 h respectively, the cytoskeletons were observed by F-actin immunostaining. (c) The quantified parameter of the percentage of F-actin area in C2C12 cells or CMs in different groups according to images in (a)-(b). Data are means ± SD, n = 6 sights per group. (d) The cell viability in C2C12 cells or CMs in different groups were assessed by CCK-8 assay. Data are means ± SD, n = 6 duplicate wells for each group. Scale bars: 20 um. ∗∗and ∗∗∗mean p < 0.01 and p < 0.001, respectively.

Article Snippet: Cell culture C2C12 mouse myoblast cell lines were purchased from ATCC. ells were cultured in Dulbecco’s modified Eagle’s medium DMEM, GIBCO) containing 10% fetal bovine serum (FBS, GIBCO), 00 U/mL penicillin and 100 μg/mL streptomycin (C2C12’s growth edium) at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Immunostaining, CCK-8 Assay

Journal: Applied Materials Today

Article Title: A biosynthesized gold nanoparticle from Staphylococcus aureus – as a functional factor in muscle tissue engineering

doi: 10.1016/j.apmt.2020.100905

Figure Lengend Snippet: Fig. 5. (a-b) The influences of the AuNPs in different concentrations on cell viabilities of C2C12 cells (a) or CMs (b) in different treatment times were systematically analysized. Data are means ± SD, n = 7 duplicate wells for each group. ∗∗∗represent p < 0.001 when compared with control. (c-d) TEM observation for the control and AuNPs-treated muscle cells. (c), C2C12 myoblasts. (d), CMs. Nu: nucleus. ES: extracellular space. Green arrows: primary lysosome. Red arrows: secondary lysosome. Purple arrows: AuNPs. PL means primary lysosome. SL means secondary lysosome.

Article Snippet: Cell culture C2C12 mouse myoblast cell lines were purchased from ATCC. ells were cultured in Dulbecco’s modified Eagle’s medium DMEM, GIBCO) containing 10% fetal bovine serum (FBS, GIBCO), 00 U/mL penicillin and 100 μg/mL streptomycin (C2C12’s growth edium) at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Control

Journal: Applied Materials Today

Article Title: A biosynthesized gold nanoparticle from Staphylococcus aureus – as a functional factor in muscle tissue engineering

doi: 10.1016/j.apmt.2020.100905

Figure Lengend Snippet: Fig. 6. The mycogenic AuNPs from S. aureus protect muscle tissues against the damage of cardiotoxin (CTX). Firstly, CMs are cultured in CMs’ growth medium for 7 days, and C2C12 myoblasts were cultured in differentiation medium for 7 days. Then, the cells were pretreated by mycogenic AuNPs for 12 h. After that, the cells were treated by CTX for another 2 h. The treatments was removed and the cells were recovered for another 7 days. Finally, the myotube’s morphology was detected by F-actin immunostaining (red) (a) and the cardiac specific proteins expression in CMs were detected by α-actinin (green) and CX-43 (red) immunostaining respectively (b). The blue color represents the nucleus. (c-d), the quantified parameters, including fusion index (c) and myotube area (d) in different groups were calculated based on images in C2C12 cells. (e-f), ɑ -actinin coverage area (e) and CX-43 coverage area (f) in different groups were calculated based on images in CMs. Column 1, control group. Column 2, AuNPs group. Column 3, AuNPs + CTX group. Column 4, CTX group. Scale bars: 20 um. Data are means ± SD. (c), n = 11, 10, 7, 14 sights for control, AuNPs, AuNPs + CTX and CTX group in 2h post-CTX; n = 7, 7,10,15 sights for control, AuNPs, AuNPs + CTX and CTX group in recovery for 7 days. (d), n = 14, 10, 8, 16 sights for control, AuNPs, AuNPs + CTX and CTX group in 2h post-CTX; n = 8, 7, 12,16 sights for control, AuNPs, AuNPs + CTX and CTX group in recovery for 7 days. (e), n = 13, 13, 7, 15 sights for control, AuNPs, AuNPs + CTX and CTX group in 2h post-CTX; n = 15, 8, 11,13 sights for control, AuNPs, AuNPs + CTX and CTX group in recovery for 7 days. (f), n = 8, 8, 7, 8 sights for control, AuNPs, AuNPs + CTX and CTX group in 2h post-CTX; n = 14, 9, 9, 12 sights for control, AuNPs, AuNPs + CTX and CTX group in recovery for 7 days. ∗, ∗∗and ∗∗∗mean p < 0.05, p < 0.01 and p < 0.001, respectively.

Article Snippet: Cell culture C2C12 mouse myoblast cell lines were purchased from ATCC. ells were cultured in Dulbecco’s modified Eagle’s medium DMEM, GIBCO) containing 10% fetal bovine serum (FBS, GIBCO), 00 U/mL penicillin and 100 μg/mL streptomycin (C2C12’s growth edium) at 37 °C in a humidified 5% CO 2 atmosphere.

Techniques: Cell Culture, Immunostaining, Expressing, Control